Gesteland, Raymond F Wills, Norma M Atkins, John F Wang, Yue Du, Zhihua Rangan, Anupama Hoffman, David W

Description

Journal ArticleMutational and NMR methods were used to investigate features of sequence, structure, and dynamics that are associated with the ability of a pseudoknot to stimulate a "1 frameshift. In vitro frameshift assays were performed on retroviral gag-pro frameshift-stimulating pseudoknots and their derivatives, a pseudoknot from the gene 32 mRNA of bacteriophage T2 that is not naturally associated with frameshifting, and hybrids of these pseudoknots. Results show that the gag-pro pseudoknot from human endogenous retrovirus-K10 (HERV) stimulates a "1 frameshift with an efficiency similar to that of the closely related retrovirus MMTV. The bacteriophage T2 mRNA pseudoknot was found to be a poor stimulator of frameshifting, supporting a hypothesis that the retroviral pseudoknots have distinctive properties that make them efficient frameshift stimulators.Ahybrid, designed by combining features of the bacteriophage and retroviral pseudoknots, was found to stimulate frameshifting while reta

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University of Utah

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Mountain West Digital Library